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1、蒲慕明所长在神经所2006年会上的讲话

新颖性(Novelty):概念或假说可以有新颖性,对自然现象有新颖的看法,就是概念的新颖性。发现新的现象也有新颖性,你做了一些探索性实验,有些自然现象以前没有人看到,你现在看到了。发现已知自然现象的新联系也算有新颖性,你发现这个现象与那个现象有关联的证据,虽然这些现象大家以前都看到,你提出这些现象之间有关联,就是新颖的发现。你也可以发明一些新的实验方法、新的分析方法,把以前人家做的东西,你用新的方式来处理、来研究、来分析,这也是新颖性。一个新颖的工作也可以是解决现在有争议的问题,虽然你没有提出新的观念或假说,但是你把有争论的问题解决了,这也是有新颖性。甚至对很老、很经典的概念,原来是大家都公认的概念,你现在有新的实验去驳倒以前那个概念,也是新颖性。
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2、Mu-ming Poo lab’s rules

2.1. Every one works at least 50 hr a week in the lab (e.g., 8+ hr a day, six days a week).
2.2. By working, I mean real bench work. This does not include surfing on the computer and sending and receiving e-mails for non-scientific matters unrelated to your work, and excessive chatting on nonscientific matters. No long lunch break except special occasions. I suggest that everyone puts in at least 6 hr concentrated bench work and 2+ hr reading and other research related activity each day.
2.3. I must be informed in person by e-mail (even in my absence from the lab) when you are absent from the lab for a whole day or more. Inform me early your vacation plan. Taking more than 20 working days out of one year is the maximum to me.
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3、RNA extraction from NATURE METHOD 2006

This protocol describes a single-step technique for the purification of RNA. Cells are homogenized in guanidinium thiocyanate and the RNA is purified from the lysate by extraction with phenol-chloroform at reduced pH.
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4、PCR引物设计及软件使用技巧

详细介绍了两种常用引物设计软件Oligo 6和 Premier Primer 5的使用方法。
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5、Gram-positive bacteria Protocol for DNA extraction

Gram-positive bacteria were lyzed for 1h at 37 oC with 300uL of lysozyme lysis buffer.
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6、SDS-PAGE protocol

详细介绍蛋白质常用电泳方法。
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7、如何防止RNA酶污染

详细介绍RNA酶污染的防护方法。
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